Journal: Diabetologia

Article Title: Increased glomerular cell (podocyte) apoptosis in rats with streptozotocin-induced diabetes mellitus: role in the development of diabetic glomerular disease.

doi: 10.1007/s00125-007-0821-y

Figure Lengend Snippet: Fig. 3 Immunohistochemistry for synaptopodin from representative non-diabetic (a) and diabetic (b) rats at 2, 4 (c, d, respectively) and 6 months (e, f, respectively) of disease duration. Vector SG (blue) was used as peroxidase substrate, ×400 magnification. g Quantification of glomerular synaptopodin expression in non-diabetic (open bars) and diabetic (closed bars) rats at 2, 4 and 6 months of disease duration. **p<0.01, ***p<0.001 vs corresponding non-diabetic controls

Article Snippet: After a 30 min incubation with StreptABComplex/HRP, sections were developed for 8– 10 min with Vector SG substrate kit for peroxidase (Vector; blue colour to distinguish from caspase-3 staining), intensified using DAB Enhancing Solution (Vector).

Techniques: Immunohistochemistry, Plasmid Preparation, Expressing

Journal: Diabetologia

Article Title: Increased glomerular cell (podocyte) apoptosis in rats with streptozotocin-induced diabetes mellitus: role in the development of diabetic glomerular disease.

doi: 10.1007/s00125-007-0821-y

Figure Lengend Snippet: Fig. 4 Immunohistochemistry for WT-1 from representative non- diabetic (a) and diabetic (b) rats at 2, 4 (c, d, respectively) and 6 months (e, f, respectively) of disease duration. DAB (brown) was used as peroxidase substrate, ×400 magnification. g Quantification of glomerular WT-1 expression in non-diabetic (open bars) and diabetic (closed bars) rats at 2, 4 and 6 months of disease duration. *p<0.05, **p<0.01 vs the corresponding non-diabetic controls

Article Snippet: After a 30 min incubation with StreptABComplex/HRP, sections were developed for 8– 10 min with Vector SG substrate kit for peroxidase (Vector; blue colour to distinguish from caspase-3 staining), intensified using DAB Enhancing Solution (Vector).

Techniques: Immunohistochemistry, Expressing

Journal:

Article Title: Antibody blockade of the Cripto CFC domain suppresses tumor cell growth in vivo

doi: 10.1172/JCI200317788

Figure Lengend Snippet: (a) Diagram of mature hCr protein: N-terminal tip (black), N-terminal region (blue), EGF-like domain (red), and CFC domain (green). Amino acid 169 is N-terminal to the GPI-linkage site. Asterisks indicate positions of mutants described in Table ​Table1.1. (b) Western blot of Cripto expressed in CHO cells (recombinant) or endogenously expressed in NCCIT and GEO tumor cell lines using A10.B2.18. (c) Immunofluorescent staining of cell surface Cripto on human breast tumor tissue with A10.B2.18 (left) or control antibody 1E6 (right). (d) Immunohistochemical staining of tumor sections with anti-Cripto mAb’s A10.B2.18 (breast and NCCIT) and B3.F6.17 (colon and GEO) or mouse IgG as a negative control. Color variations result from the use of different substrates, either Vector NovaRED or DAB substrate kits (Vector Laboratories Inc.).

Article Snippet: Color was developed using Vector NovaRED or DAB substrate kits (Vector Laboratories Inc.).

Techniques: Western Blot, Recombinant, Staining, Immunohistochemical staining, Negative Control, Plasmid Preparation